Services
Serotyping of E. coli strains
O serotyping performed using antisera produced against serogroups designated O1-O181 with the exceptions of O31, O47, O72, O93, O94, O121 as these are not designated.
H typing performed by PCR-RFLP analysis (Polymerase Chain Reaction- Restriction Fragment Length Polymorphism) of fliC gene responsible for flagella
Virulent Testing by PCR
Detection of different pathogenic types of E. coli
- Enterohemorrhagic E. coli (EHEC) or Shiga-toxin producing E. coli (STEC)
- Enteropathogenic E. coli (EPEC)
- Enterotoxigenic E. coli (ETEC)
- Enteroinvasive E. coli (EIEC)
- Enteroaggregative E. coli (EAEC)
- Necrotoxigenic E. coli (NTEC)
- Extraintestinal Pathogenic E. coli (Ex-PEC)
Detection of genes encoding virulence factors: toxins
- Shiga toxins I and II: Stx1 and StxII
- Heat Stable toxins a and b: STa and STb
- Heat-labile toxin: LT
- Cytotoxic Necrotizing Factors 1 and 2: CNF-1 and CNF-2
- Hemolysins: HlyA, HlyD, HlyE
Detection of genes encoding virulence factors: fimbriae/adhesins
- K88 (F4)
- K99 (F5)
- 987P (F6)
- F107 (F18)
- F1845
- CS31a
- BFP
- EAE
- TIR
Detection of genes encoding virulence factors for avian sp.
- Iss
- Tsh
- K1
- HlyE
Detection of genes encoding virulence factors
- O157
- H7
- Adhesins associated with EXPEC strains
Special services offered
- Gene sequencing
- ERIC-BOX PCR
- PFGE
- Anitmicrobial susceptibility
- E. coli surveillance
- E. coli monitoring