Services
Serotyping of E. coli strains
O serotyping performed using antisera produced against serogroups designated O1-O181 with the exceptions of O31, O47, O72, O93, O94, O122 as these are not designated.
H typing performed by PCR-RFLP analysis (Polymerase Chain Reaction- Restriction Fragment Length Polymorphism) of fliC gene responsible for flagella
Virulent Testing by PCR
Detection of different pathogenic types of E. coli
- Enterohemorrhagic E. coli (EHEC) or Shiga-toxin producing E. coli (STEC)
- Enteropathogenic E. coli (EPEC)
- Enterotoxigenic E. coli (ETEC)
- Enteroinvasive E. coli (EIEC)
- Enteroaggregative E. coli (EAEC)
- Necrotoxigenic E. coli (NTEC)
- Extraintestinal Pathogenic E. coli (Ex-PEC)
Detection of genes encoding virulence factors: toxins
- Shiga toxins I and II: Stx1 and StxII
- Heat Stable toxins a and b: STa and STb
- Heat-labile toxin: LT
- Cytotoxic Necrotizing Factors 1 and 2: CNF-1 and CNF-2
- Hemolysins: HlyA, HlyD, HlyE
Detection of genes encoding virulence factors: fimbriae/adhesins
- K88 (F4)
- K99 (F5)
- 987P (F6)
- F107 (F18)
- F1845
- CS31a
- BFP
- EAE
- TIR
Detection of genes encoding virulence factors for avian sp.and ExPEC
- Iss
- IutA
- IroN
- HlyF
- OmpT
- Adhesins associated with ExPEC
Detection of genes to determine O types and H types
- O157, O145, O121, O113, O111, O103, O45, O26
- H7
Special services offered
- Gene sequencing
- ERIC-BOX PCR
- PFGE (please call)
- Anitmicrobial susceptibility
- E. coli surveillance
- E. coli monitoring
SERVICES AND FEES
| Services Offered | Price/Isolate for non-profit |
Price/isolate for profit org |
|---|---|---|
| O serotyping | $20 | $35 |
| H Typing | $20 | $35 |
| Fimbrial and Toxin genes (PCR) | $50 | $70 |
| Complete Typing (O, H, Toxin and Fimbriae) | $75 | $100 |
| Individual PCR assays | $10 | $15 |
| ERIC- or BOX- or REP- PCR | Please call | Please call |
| Antimicrobial Sensitivity | $30 | $50 |
| Pulsed-Field Gel Electrophoresis | Please Call | Please call |
| Sequencing Gene of Interest | $30 | $50 |
| E.coli monitoring of animals | $15 | $15 |
| E.coli surveillance | Please Call | Please call |